All functions

DEGs_Group()

return DEGs UP and DOWN list based on intersection or union of comparisons

DEGs_RP()

return DEGs UP and DOWN list based on Rank Product

ccle_2_wide()

Convert CCLE data from long data to wide data.

ccle_crc_5

RNA-seq TPM data of 5 CRC cell line samples from CCLE.

de_analysis()

DE analysis pipeline

filter_subset_sig()

Filter specific cell type signature genes against other subsets.

get_de_table()

Get DE analysis result table(s) with statistics

get_degs()

Get differentially expressed genes by comparing specified groups

get_gsc_sig()

Collect genes from MSigDB or provided GeneSetCollection.

get_lm_sig()

Extract specific subset markers from LM7 or/and LM22

get_panglao_sig()

Extract immune subset markers from PanglaoDB website.

gls2gsc()

Convert gene-set list into GeneSetCollection

gsc_plot()

Make upset plot for given gene sets

im_data_6

RNA-seq TMM normalized counts data of 6 sorted immune subsets.

list_panglao_organs()

Show the summary info of available organs in PanglaoDB.

list_panglao_types()

Show the summary info of available cell types in PanglaoDB.

lm22

LM22 matrix for CIBERSORT.

lm7

LM7 matrix for CIBERSORT.

merge_markers()

Merge markers list into one.

msigdb_gobp_nk

Sub-collection of MSigDB gene sets.

nk_markers

NK cell markers combination.

pca_matrix_plot()

Make a matrix plot of PCA with top PCs

pca_matrix_plot_init()

Make a matrix plot of PCA with top PCs

plotPCAbiplot()

Single PCA plot function

plot_diagnostics()

plot diagnostics before and after process_data()

plot_mean_var()

plot Mean-variance trend after voom and after final linear fit

process_data()

process data

pseudo_sample_list()

Split cells according to specific factors

pseudo_samples()

Aggregate single cells to pseudo-samples according to specific factors

remove_bg_exp()

Remove markers with high signal in background data.

remove_bg_exp_mat()

Remove genes show high signal in the background expression data from markers.

select_sig()

select DEGs from multiple comparisons

sig_boxplot()

Boxplot of median expression or scores of signature

sig_gseaplot()

Visualize GSEA result with input list of gene symbols.

sig_heatmap()

Heatmap original markers and screened signature

sig_rankdensity_plot()

Plot rank density

sig_scatter_plot()

Scatter plot of signature for specific subset vs others

voom_fit_treat()

return DGEList containing vfit by limma::voom (if normalize = TRUE) and tfit by limma::treat