All functions |
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return DEGs UP and DOWN list based on intersection or union of comparisons |
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return DEGs UP and DOWN list based on Rank Product |
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Convert CCLE data from long data to wide data. |
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RNA-seq TPM data of 5 CRC cell line samples from CCLE. |
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DE analysis pipeline |
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Filter specific cell type signature genes against other subsets. |
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Get DE analysis result table(s) with statistics |
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Get differentially expressed genes by comparing specified groups |
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Collect genes from MSigDB or provided GeneSetCollection. |
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Extract specific subset markers from LM7 or/and LM22 |
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Extract immune subset markers from PanglaoDB website. |
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Convert gene-set list into GeneSetCollection |
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Make upset plot for given gene sets |
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RNA-seq TMM normalized counts data of 6 sorted immune subsets. |
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Show the summary info of available organs in PanglaoDB. |
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Show the summary info of available cell types in PanglaoDB. |
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LM22 matrix for CIBERSORT. |
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LM7 matrix for CIBERSORT. |
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Merge markers list into one. |
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Sub-collection of MSigDB gene sets. |
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NK cell markers combination. |
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Make a matrix plot of PCA with top PCs |
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Make a matrix plot of PCA with top PCs |
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Single PCA plot function |
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plot diagnostics before and after |
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plot Mean-variance trend after voom and after final linear fit |
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process data |
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Split cells according to specific factors |
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Aggregate single cells to pseudo-samples according to specific factors |
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Remove markers with high signal in background data. |
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Remove genes show high signal in the background expression data from markers. |
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select DEGs from multiple comparisons |
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Boxplot of median expression or scores of signature |
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Visualize GSEA result with input list of gene symbols. |
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Heatmap original markers and screened signature |
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Plot rank density |
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Scatter plot of signature for specific subset vs others |
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return DGEList containing vfit by limma::voom (if normalize = TRUE) and tfit by limma::treat |